In the present study, the volatile compounds of aerial parts of Salvia officinalis L. (sage) were extracted and analysed by gas chromatography-mass spectrometry (GC-MS) using the Nist and Willey libraries. It was determined that the main components of sage were camphor  (26.05%), α-thujone (17.46%), 1.8-cineole (12.11%), viridiflorol (6.95%) and β-thujone (3.57%). Then, antimicrobial activity of essential oil of sage against Escherichia coli (ATCC 25293), Bacillus subtilis (ATCC 6633), Staphylococcus aureus (ATCC 25925), Pseudomonas aureginosa, Candida albicans and Candida parapsilosis were examined by using spectrophotometric broth microdilution method. Accordingly, the highest MIC value of S. officinalis essential oil was against S. aureus (11.7 µg/mL), while the lowest activity was P. aureginosa (109.9 µg/mL). Furthermore, MIC values ​​for the other microorganisms were determined as 11.8 µg/mL for B. subtilis, 59.0 µg/mL for E. coli, 25.3 µg/mL for C. albicans and 15.4 µg/mL for C. parapsilosis. As a result, essential oil of aerial parts of S. officinalis were noted to be high antimicrobial efficiency under concentration of 110 µg/mL.