The deleted in azoospermia-like () gene encoding an RNA binding protein is pivotal in gametogenesis in lots of species and also acts as a pre-meiosis marker. The current study was conducted to detect expression profiles and single nucleotide polymorphisms (SNPs) of in sheep using qPCR, DNA-pooled sequencing, improved multiplex ligase detection reaction (iMLDR) and restriction fragment length polymorphism (RFLP) methods. The results confirmed that ovine showed the highest expression level at six-months of age across five developmental stage. At six-month stage, expressed primarily in testis across seven tissues analyzed. The abundance of in the large-testis group is higher than that in the small-testis group although it is not significant. In addition, six SNPs (SNP1-SNP6) were identified in . Of those, SNP1 ( < 0.05) and SNP6 ( < 0.01) were significantly correlated with the variation coefficient between left and right epididymis weight (VCTW). The current study implies may play important roles in testicular development and its SNPs are associated with testicular parameters, which supply important indicators for ram selection at early stage.