The aim of this study is to investigate the relationship between bioclimatic comfort and land use in Trabzon by using geographical information systems and remote sensing technologies. We aimed to evaluate the relationship between the bioclimatic conditions of the years 1985, 1994, 2005, and 2018 with the use of land in the same years in the province of Trabzon in seasonal and annual periods. Physiological equivalent temperature (PET) index, which takes into consideration the physiological characteristics of humans, was used when determining bioclimatic comfort zones. The meteorological parameters used in the calculation of this index are as follows: daily average temperature values reduced to sea level, daily average relative humidity, and wind speed. The inverse distance weighting (IDW) method was preferred in the calculation of the spatial distribution of the obtained values at sea level. Using DEM data, height-dependent PET values were obtained and bioclimatic comfort maps were generated. According to the years of the bioclimatic comfort maps produced, land use maps were created by using CORINE land cover data. Then, the relationship between bioclimatic comfort zones and land use was examined.