Lesbian, gay, bisexual and trans (LGBT) parents may have negative experiences when accessing healthcare for their children as a result of homophobia, transphobia or other forms of prejudice. Patients and their families have reported receiving inappropriate comments from healthcare professionals about sexual orientation, despite policy and government recommendations advising healthcare professionals to provide care that demonstrates respect, dignity and compassion.The aim of this pilot study was to understand the experience of non-biological lesbian mothers accessing healthcare for their children and the types of attitudes and language they encountered. The pilot was also carried out to determine that the style of questioning allowed differentiation between sexuality and gender.This narrative enquiry pilot study used interviews conducted with three non-biological lesbian mothers aged 36-45 years. Two of the participants were a married couple with adopted children; the other had undergone in vitro fertilisation (IVF) with her partner. Of the three participants, two were the authors of this study. The data were coded using thematic analysis.The participants' interviews revealed mixed experiences with healthcare professionals' attitudes. Four common themes emerged: attitudes and managing healthcare experiences; acknowledgement of sexual orientation; professional standards; and family constellation.The study found that non-biological lesbian mothers experience positive and negative interactions with healthcare professionals but feel disenfranchised because of their non-biological status. A reason for the negative interactions could be a lack of training for healthcare professionals about the challenges that lesbian mothers face when accessing healthcare for their children.