Excessive reactive oxygen species (ROS) may attack biological macromolecules and induce oxidative stress. The inhibition by ascorbic acid (AsA) on oxidative damage has been reported in fruits, while the barrier effect of ceramide has also been proved. However, there are few reports about the effects of ceramide-AsA interactions to enhance storability and boost antioxidant systems in fruits during storage. This study was conducted to study the synergistic effects of AsA in combination with ceramide on the quality of postharvest strawberries (Fragaria anannasa cv. Tianbao).Treatment with 100 mg L AsA plus 1.2 mmol L ceramide significantly delayed the rot of strawberry, reduced the water loss and the contents of ROS, malonaldehyde (MDA), and proline, however, increased the contents of total flavonoids, total phenols, and anthocyanins compared with other treatments. Also, treatment with 100 mg L AsA plus 1.2 mmol L ceramide significantly increased the activities of peroxidase (POD) and superoxide dismutase (SOD) but inhibited the activity of polyphenol oxidase (PPO).It suggested that treatment with 100 mg L AsA plus 1.2 mmol L ceramide could significantly reduce the oxidative damage and maintain the storage quality of strawberry during storage by enhancing the antioxidant systems. This article is protected by copyright. All rights reserved.