The present work analyses the chromatographic profile of the peels from fruits of different citrus cultivated in Colombia: sweet orange ( [L.] Osbeck var. Valencia), mandarins ( L. var. Arrayana and Oneco), Key lime ( [Christ.] Swingle var. Pajarito), Mandarine lime (, a hybrid between and ) and Tahitian lime ( Tanaka, Persian lime). Coumarins, furanocoumarins and polymethoxylated flavones are the major compounds. Then, six coumarins were isolated and identified from fruits of Tahitian and Key lime corresponding to 5-geranyloxy-7-methoxycoumarin; 5,7-dimethoxycoumarin (. limettin); 5,8-dimethoxypsoralen ( isopimpinellin); 5-methoxypsoralen (. bergaptene); 5-geranoxypsoralen ( bergamottin) and 5-(2,3-dihydroxy-3-methylbutoxy) psoralen ( oxypeucedanin hydrate). Coumarins and furanocoumarins were quantified by liquid chromatography (HPLC-DAD). Results show that the prenylated compounds were present in high concentrations in Tahitian and Key lime but in very low amounts in mandarins and sweet orange. Subsequently, the antifungal activity (inhibition of mycelial growth and germination of spores) of the coumarins against the fungus causing the anthracnose, sp. (isolated from aerial parts of Tahitian lime) was determined. The compounds limettin and bergaptene, as well as mixtures of them, showed significant inhibitory effect (radial growth and spore germination) when compared to the control. Finally, the effect of some recognized elicitors to induce the coumarin production in fruits of was evaluated. The results showed that the chemical profiles are dependent on the applied elicitor and the post-induction time. As a result of the induction, a high concentration of some coumarins and furanocoumarins was maintained in the course of time for the Tahitian lime. In conclusion, isolated coumarins could be involved in the defense mechanisms of , and and their accumulation may be modulated by the application of elicitors.