sp. nov., isolated from a crocodile pond.

sp. nov., isolated from a crocodile pond.

Chen, Wen-Ming;Xie, Yi-Ru;Sheu, Der-Shyan;Tsai, Jyh-Ming;Sheu, Shih-Yi;
international journal of systematic and evolutionary microbiology 2020 Vol. 70 pp. 5141-5148
108
chen2020international

Abstract

Bacterial strain CCP-6, isolated from a freshwater pond in Taiwan, was characterized using a polyphasic taxonomy approach. Phylogenetic analyses based on 16S rRNA gene sequences and an up-to-date bacterial core gene set (92 protein clusters) indicated that strain CCP-6 is affiliated with species in the genus . Strain CCP-6 was most closely related to CCUG 44693 with a 98.9% 16S rRNA gene sequence similarity. Cells were Gram-stain-negative, aerobic, non-motile, rod-shaped and formed light pink-coloured colonies. Optimal growth occurred at 30 °C, pH 6 and in the absence of NaCl. The major fatty acids of strain CCP-6 were C7, C and C cyclo 8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylmethylethanolamine, diphosphatidylglycerol, three unidentified aminophospholipids and an unidentified phospholipid. The predominant polyamine was spermidine. The major isoprenoid quinone was Q-10. The DNA G+C content of the genomic DNA was 69.3 mol%. Strain CCP-6 showed 85.8% average nucleotide identity and 14.5% digital DNA-DNA hybridization identity with CCUG 44693. On the basis of the genotypic, chemotaxonomic and phenotypic data, strain CCP-6 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain is CCP-6 (=BCRC 81095=LMG 30310=KCTC 62188).

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